Correlating macrophage morphology and cytokine production resulting from biomaterial contact
Identifieur interne : 001613 ( Main/Exploration ); précédent : 001612; suivant : 001614Correlating macrophage morphology and cytokine production resulting from biomaterial contact
Auteurs : Hyun-Su Lee [États-Unis] ; Stanley J. Stachelek [États-Unis] ; Nancy Tomczyk [États-Unis] ; Matthew J. Finley [États-Unis] ; Russell J. Composto [États-Unis] ; David M. Eckmann [États-Unis]Source :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2013-01.
English descriptors
- Teeft :
- Actin, Adherent, Adherent cell, Adherent cells, Adherent macrophages, Amoeboid, Article figure, Assay, Biocompatibility, Biomaterial, Biomaterials, Biomed, Biomed mater, Biomedical materials research, Blot analysis, Cell biol, Cell density, Cell morphology, Cell surface area, Cell volume, Chimeric protein, Chitosan, Composto, Confocal, Cytokine, Cytokine secretion, Cytoplasmic surface, Eckmann, Foreign body reaction, Hyaluronic acid, Lentiviral vector, Macrophage, Macrophage adhesion, Macrophage morphology, Mater, Monocyte, Morphological response, Morphological responses, Morphology, Online issue, Polymer, Polyurethane, Quaternary ammonium salts, Round morphology, Secretion, Secretion levels, Shape factor, Standard deviation, Surface area, Surface density, Surface type, Surface types, Titanium surface topography, Transduced, Transduced cells, Uorescence.
Abstract
The morphological and inflammatory responses of adherent macrophages are correlated to evaluate the biocompatibility of surfaces. Monocyte‐derived macrophage (MDM), THP‐1, and THP‐1 cells expressing GFP‐actin chimeric protein were seeded onto glass, polyurethane (PU), and glass surface modified with quaternary ammonium salt functionalized chitosan (CH‐Q) and hyaluronic acid (HA). Using confocal microscopy, the surface area, volume and 3D shape factor of adherent macrophages was quantified. For comparison, functional consequences of cell‐surface interactions that activate macrophages and thereby elicit secretion of a proinflammatory cytokine were evaluated. Using an enzyme linked immune sorbent assay, tumor necrosis factor‐alpha (TNF‐α) was measured. On glass, macrophages exhibited mainly an amoeboid shape, exhibited the largest surface area, volume, and 3D shape factor and produced the most TNF‐α. On PU, macrophages displayed mainly a hemispherical shape, exhibited an intermediate volume, surface area and 3D shape factor, and produced moderate TNF‐α. In contrast, on CH‐Q and HA surfaces, macrophages were spherical, exhibited the smallest volume, surface area, and 3D shape factor, and produced the least TNF‐α. These studies begin to validate the use of GFP‐actin‐modified MDM as a novel tool to correlate cell morphology with inflammatory cell response. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:203–212, 2013.
Url:
DOI: 10.1002/jbm.a.34309
Affiliations:
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Monocyte‐derived macrophage (MDM), THP‐1, and THP‐1 cells expressing GFP‐actin chimeric protein were seeded onto glass, polyurethane (PU), and glass surface modified with quaternary ammonium salt functionalized chitosan (CH‐Q) and hyaluronic acid (HA). Using confocal microscopy, the surface area, volume and 3D shape factor of adherent macrophages was quantified. For comparison, functional consequences of cell‐surface interactions that activate macrophages and thereby elicit secretion of a proinflammatory cytokine were evaluated. Using an enzyme linked immune sorbent assay, tumor necrosis factor‐alpha (TNF‐α) was measured. On glass, macrophages exhibited mainly an amoeboid shape, exhibited the largest surface area, volume, and 3D shape factor and produced the most TNF‐α. On PU, macrophages displayed mainly a hemispherical shape, exhibited an intermediate volume, surface area and 3D shape factor, and produced moderate TNF‐α. In contrast, on CH‐Q and HA surfaces, macrophages were spherical, exhibited the smallest volume, surface area, and 3D shape factor, and produced the least TNF‐α. These studies begin to validate the use of GFP‐actin‐modified MDM as a novel tool to correlate cell morphology with inflammatory cell response. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:203–212, 2013.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Lee, Hyun U" sort="Lee, Hyun U" uniqKey="Lee H" first="Hyun-Su" last="Lee">Hyun-Su Lee</name></region><name sortKey="Composto, Russell J" sort="Composto, Russell J" uniqKey="Composto R" first="Russell J." last="Composto">Russell J. Composto</name><name sortKey="Composto, Russell J" sort="Composto, Russell J" uniqKey="Composto R" first="Russell J." last="Composto">Russell J. Composto</name><name sortKey="Eckmann, David M" sort="Eckmann, David M" uniqKey="Eckmann D" first="David M." last="Eckmann">David M. Eckmann</name><name sortKey="Eckmann, David M" sort="Eckmann, David M" uniqKey="Eckmann D" first="David M." last="Eckmann">David M. Eckmann</name><name sortKey="Eckmann, David M" sort="Eckmann, David M" uniqKey="Eckmann D" first="David M." last="Eckmann">David M. Eckmann</name><name sortKey="Eckmann, David M" sort="Eckmann, David M" uniqKey="Eckmann D" first="David M." last="Eckmann">David M. Eckmann</name><name sortKey="Finley, Matthew J" sort="Finley, Matthew J" uniqKey="Finley M" first="Matthew J." last="Finley">Matthew J. Finley</name><name sortKey="Lee, Hyun U" sort="Lee, Hyun U" uniqKey="Lee H" first="Hyun-Su" last="Lee">Hyun-Su Lee</name><name sortKey="Lee, Hyun U" sort="Lee, Hyun U" uniqKey="Lee H" first="Hyun-Su" last="Lee">Hyun-Su Lee</name><name sortKey="Stachelek, Stanley J" sort="Stachelek, Stanley J" uniqKey="Stachelek S" first="Stanley J." last="Stachelek">Stanley J. Stachelek</name><name sortKey="Tomczyk, Nancy" sort="Tomczyk, Nancy" uniqKey="Tomczyk N" first="Nancy" last="Tomczyk">Nancy Tomczyk</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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